rabbit anti‐enc1 15007‐1‐ap  (Proteintech)

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: High expression of ENC1 in lung cancer tissues and cells. (A and B) The expression of ENC1 between lung cancer and para-cancerous tissue from the TCGA database. (C) Kaplan-Meier survival plot of overall survival of lung cancer in TCGA database, categorized according to ENC1 gene expression (high vs. low, based on mean expression). (D) The expression of ENC1 in normal and tumor tissues by RT-qPCR. (E) The expression of ENC1 in tumor tissues between stage II and III by RT-qPCR. (F) The expression of ENC1 in normal and tumor tissues by immunohistochemical analysis. (G and H) The expression of ENC1 between normal cell line and lung cancer cell lines by RT-qPCR and western blot analysis. Scale bar, 50 µ m; * P<0.05, *** P<0.001 vs. normal group; **P<0.01 vs. 16HBE cell group; ENC1, ectodermal-neural cortex 1.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Associations between ENC1 expression and the clinicopathological parameters of patients with lung adenocarcinoma.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Expressing

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Gene expression profiling of si-ENC1-transfected A549. (A) Volcano plot of all genes detected in transcriptome analysis. (B) Heatmap represents the magnitude of gene expression of 1404 genes (P<0.05, LogFC ≥1 o r≤−1). (C and D) Relative fold change of genes representing key pathways identified by GO and KEGG analyses. (E and F) Heatmap represents magnitude of gene expression of cell adhesion and regulation of cell binding. ENC1, ectodermal-neural cortex 1.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Expressing, Transfection, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the migration and invasion of NSCLC cells. (A) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C-J) The effects of ENC1 deficiency on cell migration and invasion were investigated by Transwell assay. (K-M) The effects of ENC1 deficiency on the expression of N-cadherin, E-cadherin, MMP2 and MMP9 were investigated by western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Migration, Quantitative RT-PCR, Western Blot, Transwell Assay, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the proliferation of NSCLC cells. (A and B) Effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 by CCK-8 assay. (C-F) The effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 using an EdU detection kit. * P<0.05, ** P<0.01 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: CCK-8 Assay

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 affects the JNK and ERK pathways. (A-C) Effects of ENC1 deficiency on the expression of p-ERK, ERK, p-JNK, JNK p-p38 and p38 were quantified in A549 and H1299 cells by western blot analysis. * P<0.05 vs. si-NC group. ENC1, ectodermal-neural cortex 1.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the growth of NSCLC in a mouse model. (A) Image of murine tissues from the 2 groups. (B) Tumor volume in the sh-ctrl and sh-ENC1 groups. (C) Tumor weight in the sh-ctrl and sh-ENC1 groups. (D) Tissue pathological changes in the sh-ctrl and sh-ENC1 groups, determined by H&E staining. (E-H) The expression levels of N-cadherin, E-cadherin, MMP2 and MMP9 were investigated and compared between the sh-ctrl and sh-ENC1 groups by immunohistochemistry. Scale bar, 50 µ m; ** P<0.01, vs. sh-ctrl. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Staining, Expressing, Immunohistochemistry

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Upregulation of ENC1 enhances the proliferation, migration and invasion of NSCLC cells. (A) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C) Effects of ENC1 overexpression on proliferation were investigated in A549 and H1299 cells by CCK-8 assay. (D) The effects of ENC1 overexpression on the proliferation were investigated in A549 and H1299 cells using an EdU detection kit. (E and F) The effects of ENC1 overexpression on cell migration and invasion were investigated by Transwell assay. * P<0.05, ** P<0.01, *** P<0.001 vs. OE-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: They were then incubated with anti-ENC1 antibody (1:200, ab124902; Abcam) overnight (at 4°C) and secondary antibody (1:200, 7074S; Cell Signaling Technology, Inc.) for 30 min (at 37°C).

Techniques: Migration, Over Expression, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Transwell Assay

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: High expression of ENC1 in lung cancer tissues and cells. (A and B) The expression of ENC1 between lung cancer and para-cancerous tissue from the TCGA database. (C) Kaplan-Meier survival plot of overall survival of lung cancer in TCGA database, categorized according to ENC1 gene expression (high vs. low, based on mean expression). (D) The expression of ENC1 in normal and tumor tissues by RT-qPCR. (E) The expression of ENC1 in tumor tissues between stage II and III by RT-qPCR. (F) The expression of ENC1 in normal and tumor tissues by immunohistochemical analysis. (G and H) The expression of ENC1 between normal cell line and lung cancer cell lines by RT-qPCR and western blot analysis. Scale bar, 50 µ m; * P<0.05, *** P<0.001 vs. normal group; **P<0.01 vs. 16HBE cell group; ENC1, ectodermal-neural cortex 1.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Associations between ENC1 expression and the clinicopathological parameters of patients with lung adenocarcinoma.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Expressing

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Gene expression profiling of si-ENC1-transfected A549. (A) Volcano plot of all genes detected in transcriptome analysis. (B) Heatmap represents the magnitude of gene expression of 1404 genes (P<0.05, LogFC ≥1 o r≤−1). (C and D) Relative fold change of genes representing key pathways identified by GO and KEGG analyses. (E and F) Heatmap represents magnitude of gene expression of cell adhesion and regulation of cell binding. ENC1, ectodermal-neural cortex 1.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Expressing, Transfection, Binding Assay

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the migration and invasion of NSCLC cells. (A) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C-J) The effects of ENC1 deficiency on cell migration and invasion were investigated by Transwell assay. (K-M) The effects of ENC1 deficiency on the expression of N-cadherin, E-cadherin, MMP2 and MMP9 were investigated by western blot analysis. * P<0.05, ** P<0.01, *** P<0.001 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Migration, Quantitative RT-PCR, Western Blot, Transwell Assay, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the proliferation of NSCLC cells. (A and B) Effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 by CCK-8 assay. (C-F) The effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 using an EdU detection kit. * P<0.05, ** P<0.01 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: CCK-8 Assay

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 affects the JNK and ERK pathways. (A-C) Effects of ENC1 deficiency on the expression of p-ERK, ERK, p-JNK, JNK p-p38 and p38 were quantified in A549 and H1299 cells by western blot analysis. * P<0.05 vs. si-NC group. ENC1, ectodermal-neural cortex 1.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Downregulation of ENC1 inhibits the growth of NSCLC in a mouse model. (A) Image of murine tissues from the 2 groups. (B) Tumor volume in the sh-ctrl and sh-ENC1 groups. (C) Tumor weight in the sh-ctrl and sh-ENC1 groups. (D) Tissue pathological changes in the sh-ctrl and sh-ENC1 groups, determined by H&E staining. (E-H) The expression levels of N-cadherin, E-cadherin, MMP2 and MMP9 were investigated and compared between the sh-ctrl and sh-ENC1 groups by immunohistochemistry. Scale bar, 50 µ m; ** P<0.01, vs. sh-ctrl. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: International Journal of Molecular Medicine

Article Title: Ectodermal-neural cortex 1 affects the biological function of lung cancer through the MAPK pathway

doi: 10.3892/ijmm.2021.4912

Figure Lengend Snippet: Upregulation of ENC1 enhances the proliferation, migration and invasion of NSCLC cells. (A) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C) Effects of ENC1 overexpression on proliferation were investigated in A549 and H1299 cells by CCK-8 assay. (D) The effects of ENC1 overexpression on the proliferation were investigated in A549 and H1299 cells using an EdU detection kit. (E and F) The effects of ENC1 overexpression on cell migration and invasion were investigated by Transwell assay. * P<0.05, ** P<0.01, *** P<0.001 vs. OE-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.

Article Snippet: Primary antibodies used in the present study included the following: ENC1 was obtained from Abcam (1:1,000, ab124902); N-Cadherin (1:1,000, 13116S), E-Cadherin (1:1,000, 3195S), matrix metalloproteinase (MMP)2 (1:1,000, 40994S), MMP9 (1:1,000, 13667S), p-extracellular signal-regulated kinase (ERK) (1:1,000, AP0485), ERK (1:1,000, 4695T), p-c-Jun N-terminal kinase (JNK) (1:1,000, 4668T), JNK (1:1,000, 9252T), p-p38 (1:1,000, 4511T), p-38 (1:1,000, 8690T) and β-actin (1:1,000, 3700T) were obtained from Cell Signaling Technology, Inc.

Techniques: Migration, Over Expression, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Transwell Assay